Difference between revisions of "Part:BBa K627011:Design"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K627011 short</partinfo> | <partinfo>BBa_K627011 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
| − | + | This biobrick was built by PCR using the following PCR primers:<br> | |
| − | + | Forward primer (p_TorA-f): <br> | |
| − | + | CTTCTAGATGAACAATAACGATCTCTTTCAGGCATC <br> | |
| − | + | Reverse primer (o_bla_igem_r): <br> | |
| + | CTACTAGTATTAACCGGTCCAATGCTTAATCAGTGAGGCAC | ||
===Source=== | ===Source=== | ||
Revision as of 14:39, 21 September 2011
Fusion part of pBAD arabinose-inducible induction system and the HRV 14_3C protease
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1523
Illegal BglII site found at 1711
Illegal BamHI site found at 1144 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1239
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961
Design Notes
This biobrick was built by PCR using the following PCR primers:
Forward primer (p_TorA-f):
CTTCTAGATGAACAATAACGATCTCTTTCAGGCATC
Reverse primer (o_bla_igem_r):
CTACTAGTATTAACCGGTCCAATGCTTAATCAGTGAGGCAC