Difference between revisions of "Part:BBa I746907:Experience"
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I746907 (T7 promotor with GFP) in pSB1A2 in BL21DE3 cells | I746907 (T7 promotor with GFP) in pSB1A2 in BL21DE3 cells | ||
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change of medium to M63 (LB shows slight fluorescence itself) | change of medium to M63 (LB shows slight fluorescence itself) | ||
| − | '''Problem''': measurement of Absorbance only every 100 minutes led to spikes in the diagram | + | '''Problem''': measurement of Absorbance only every 100 minutes led to spikes in the diagram (Fluorescence measured every 10 min.) |
====results==== | ====results==== | ||
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for this diagram: | for this diagram: | ||
| − | * | + | * calculation: Fluorescence/Absorbance |
* mean of triplicate measurement | * mean of triplicate measurement | ||
* adjusted to 100 % | * adjusted to 100 % | ||
Revision as of 14:27, 21 September 2011
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_I746907
User Reviews
TU_Munich Team 2011
We tested the part and it worked very well.
TU_Munich Team 2011 GFP Assay
I746907 (T7 promotor with GFP) in pSB1A2 in BL21DE3 cells
change of medium to M63 (LB shows slight fluorescence itself)
Problem: measurement of Absorbance only every 100 minutes led to spikes in the diagram (Fluorescence measured every 10 min.)
results
no concentration dependency of GFP induction as IPTG concentration was in saturation range
for this diagram:
- calculation: Fluorescence/Absorbance
- mean of triplicate measurement
- adjusted to 100 %
- corrected for outliers
- shortened to 700 min
UNIQ31b1fbc954c418a1-partinfo-00000000-QINU
UNIQ31b1fbc954c418a1-partinfo-00000001-QINU