Difference between revisions of "Part:BBa K676011:Experience"

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[[Image:Spy.jpg]]
 
[[Image:Spy.jpg]]
  
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According to our result in the 1D chloroquine gel electrophoresis (at 2.5 μg/mL), the plasmid with the Mu phage GBS (on pSB1C3 backbone) migrated further compared to the control plasmid (pSB1C3 with RFP expression cassette - BBa_J04450). At the chloroquine concentration which we used, the more negatively supercoiled plasmid will migrate further while the more relaxed molecules will travel slower through the gel.  
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According to our result in the 1D chloroquine gel electrophoresis (at 2.5 μg/mL), the plasmid with the pSC101 GBS (on pSB1C3 backbone) migrated further compared to the control plasmid (pSB1C3 with RFP expression cassette - BBa_J04450). At the chloroquine concentration which we used, the more negatively supercoiled plasmid will migrate further while the more relaxed molecules will travel slower through the gel.  
  
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Besides achieving a higher level of supercoiling, the quality of the supercoiled plasmids also showed to be better. The uniformity or homogeneity of the supercoiled target plasmids increases by close to 40% compared to the control.
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Besides achieving a higher level of supercoiling, the quality of the supercoiled plasmids also showed to be better. The uniformity or homogeneity of the supercoiled target plasmids increases by close to 15% compared to the control due to the pSC101 GBS.
  
 
===User Reviews===
 
===User Reviews===

Revision as of 13:47, 21 September 2011

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K676011

Spy.jpg

According to our result in the 1D chloroquine gel electrophoresis (at 2.5 μg/mL), the plasmid with the pSC101 GBS (on pSB1C3 backbone) migrated further compared to the control plasmid (pSB1C3 with RFP expression cassette - BBa_J04450). At the chloroquine concentration which we used, the more negatively supercoiled plasmid will migrate further while the more relaxed molecules will travel slower through the gel.

Besides achieving a higher level of supercoiling, the quality of the supercoiled plasmids also showed to be better. The uniformity or homogeneity of the supercoiled target plasmids increases by close to 15% compared to the control due to the pSC101 GBS.

User Reviews

UNIQf548085dcd70b4d5-partinfo-00000000-QINU UNIQf548085dcd70b4d5-partinfo-00000001-QINU