Difference between revisions of "Part:BBa K564014"

Latest revision as of 13:17, 21 September 2011

atC-->chiX

This part contains a anhydrotetracycline regulatable promoter and the gene for chiX downstream of it. Upon aTC addition, it triggers expression of the sRNA chiX.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 848
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 838
1000
COMPATIBLE WITH RFC[1000]

Revision as of 23:44, 20 September 2011 (view source) Alewinska88 (Talk \| contribs) ← Older edit		Latest revision as of 13:17, 21 September 2011 (view source) Alewinska88 (Talk \| contribs)
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	<partinfo>BBa_K564014 short</partinfo>		<partinfo>BBa_K564014 short</partinfo>

−	This ~~device is based on~~ gene ~~encoding small RNA (sRNA), which represses ChiP gene synthesis by pairing with a sequence in 5’ untranslated region (UTR) near ribosome binding site (RBS)~~ of ~~chiP mRNA~~. ~~This leads to degradation~~ of the ~~targeted mRNA by enzymes called RNases.The repression occurs in the absence of the substrates (chitooligosaccharides) stimulating chiP gene transcription. It is fused with Ptet promoter~~.	+	This part contains a anhydrotetracycline regulatable promoter and the gene for chiX downstream of it. Upon aTC addition, it triggers expression of the sRNA chiX.