Difference between revisions of "Part:BBa K568003"
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[[Image:TUM_2011_Miller_Assay_15.9.2011_of_BBa_K568003.jpg|left|thumb|550px|Results of the Miller Assay of BBa_K568003. The part was transcribed by genome-coded T7 polymerase, which was induced by addition of varying concentrations of IPTG. The control strain was not able to produce T7 polymerase.]] | [[Image:TUM_2011_Miller_Assay_15.9.2011_of_BBa_K568003.jpg|left|thumb|550px|Results of the Miller Assay of BBa_K568003. The part was transcribed by genome-coded T7 polymerase, which was induced by addition of varying concentrations of IPTG. The control strain was not able to produce T7 polymerase.]] | ||
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| + | ===Sequence and Features=== | ||
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| + | <span class='h3bb'>Sequence and Features</span> | ||
| + | <partinfo>BBa_K568003 SequenceAndFeatures</partinfo> | ||
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| + | <!-- Uncomment this to enable Functional Parameter display | ||
| + | ===Functional Parameters=== | ||
| + | <partinfo>BBa_K568003 parameters</partinfo> | ||
| + | <!-- --> | ||
Revision as of 12:51, 21 September 2011
T7 promoter lacZ reporter part
beta-galactosidase expression upon T7 polymerase binding
Usage and Biology
This part can be used as a reporter plasmid. It expresses beta-galactosidase if there is a T7 polymerase (or a mutant variant) nearby or transcribed through another part. The polymerase binds to the T7 promoter and transcription of the lacZ-Gene can occur, leading to beta-galactosidase expression.
Experimental Testing
The part was tested by the 2011 team of the TU_Munich on the 25th of September 2011. For this purpose, a Miller Assay was conducted. The results can be seen below.
Sequence and Features
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]