Difference between revisions of "Part:BBa K568000"
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<partinfo>BBa_K568000 short</partinfo>
 
<partinfo>BBa_K568000 short</partinfo>
  
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Single red light sensor without additional genes. This part can pe clonend anywhere one wants to.  
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Single red light inducible construct without downstream genes. This part can be cloned in front of any desired gene product.  
  
  
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<partinfo>BBa_K568000 parameters</partinfo>
 
<partinfo>BBa_K568000 parameters</partinfo>
  
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Induction with red light at 705 nm
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Induction with red light at 705 nm.
  
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Since sensor is constitutive switched on, red light signalling can be shut down with 650 nm.
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Since OmpR is expressed phosphorylated and therefore gene expression is induced in the dark, red light signalling can be shut down with 650 nm.[1]

Revision as of 12:38, 21 September 2011

Red light sensor

Single red light inducible construct without downstream genes. This part can be cloned in front of any desired gene product.


Usage and Biology

Red light induces the autophosphorylation at the cytosolic site of cph8. This leads to phosphorylation of OmpR which subsequently binds to OmpC promotor and enables transcription of a following gene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 644
    Illegal XhoI site found at 1786
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 971
  • 1000
    COMPATIBLE WITH RFC[1000]


Functional Parameters

n/aRed light sensor

Induction with red light at 705 nm.

Since OmpR is expressed phosphorylated and therefore gene expression is induced in the dark, red light signalling can be shut down with 650 nm.[1]