Difference between revisions of "Part:BBa K564004"
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| − | ChiP encodes enterobacterial chitoporin required for uptake of chitin-derived oligosaccharides. This outer membrane porin is required for Salmonella and E.coli growth on chitooligosaccharides as a sole sources of both carbon and nitrogen. It is only synthesized in the presence of these substrates. In nature it is subject | + | ChiP encodes an enterobacterial chitoporin required for uptake of chitin-derived oligosaccharides. This outer membrane porin is required for Salmonella and E.coli growth on chitooligosaccharides as a sole sources of both carbon and nitrogen. It is only synthesized in the presence of these substrates. In nature it is subject to post-transcriptional regulation by sRNA chiX (also known as micM or sroB) which pairs to its RBS region, preventing translation and triggering chiP mRNA destabilisation. |
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Latest revision as of 12:06, 21 September 2011
ChiP promoter and 5' UTR; WT RBS (AAGAGG)
ChiP encodes an enterobacterial chitoporin required for uptake of chitin-derived oligosaccharides. This outer membrane porin is required for Salmonella and E.coli growth on chitooligosaccharides as a sole sources of both carbon and nitrogen. It is only synthesized in the presence of these substrates. In nature it is subject to post-transcriptional regulation by sRNA chiX (also known as micM or sroB) which pairs to its RBS region, preventing translation and triggering chiP mRNA destabilisation.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]