Difference between revisions of "Part:BBa K564013"

Revision as of 11:59, 21 September 2011

Upstream mutated chitoporin part fused with lacZ

ChiP encodes enterobacterial chitoporin required for uptake of chitin-derived oligosaccharides. This outer membrane porin is required for Salmonella and E.coli growth on chitooligosaccharides as a sole source of both carbon and nitrogen. It is only synthesized in the presence of these substrates. In nature it is regulated by sRNAs. In this biobrick we altered the ribosome binding site for ChiP regulator – ChiX (AAGAGG-->AGGAGA). It was done in order to show that the regulatory system depends on the specific base pairing between ChiP and its regulator. The promoter and reporter have not been altered compared to K564012.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K564013 short</partinfo>
-ChiP encodes enterobacterial chitoporin required for uptake of chitin-derived oligosaccharides. This outer membrane porin is required for Salmonella and E.coli growth on chitooligosaccharides as a sole source of both carbon and nitrogen. It is only synthesized in the presence of these substrates. In nature it is regulated by sRNAs. In this biobrick we altered the binding site for ChiP regulator – ChiX. It was done in order to show that the regulatory system depends on the specific base pairing between ChiP and its regulator. The upstream part of this gene is fused with reporter gene lacZ to allow checking if the regulation works correctly.
+ChiP encodes enterobacterial chitoporin required for uptake of chitin-derived oligosaccharides. This outer membrane porin is required for Salmonella and E.coli growth on chitooligosaccharides as a sole source of both carbon and nitrogen. It is only synthesized in the presence of these substrates. In nature it is regulated by sRNAs. In this biobrick we altered the ribosome binding site for ChiP regulator – ChiX (AAGAGG-->AGGAGA). It was done in order to show that the regulatory system depends on the specific base pairing between ChiP and its regulator. The promoter and reporter have not been altered compared to K564012.
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