Difference between revisions of "Part:BBa K676001:Design"
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Revision as of 11:26, 21 September 2011
Gyrase Binding Site from Mu Bacteriophage
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 191
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The gyrase binding sites (GBS) or strong gyrase sites and are found to be about 280bp ( Mu phage SGS) according to Oram(2003). In the 280bp DNA sequence, approximately 130bp will be wrapped around the tetrameric enzyme in a way similar to nuclesome formation.
By using bioinformatics software and websites such as NCBI , eurofins operons and the Mu GBS sequence from Oram (2003) , we have designed the appropriate forward and reverse primers to extract and amplify the 280 bp sequence from the Mu phage DNA template which was kindly provided by the Department of Molecular Biosciences .
Source
Wild type Mu bacteriophage genome