Difference between revisions of "Part:BBa K592004"
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YF1 is a fusion protein of YtvA (''B subtilis'') and FixL (''B japonicum''). It contains the N-terminal region of YtvA (1-127) and C-terminal region of FixL (258-505), interconnected by a helical linker. This fusion protein is designed by Andreas Moglich and Keith Moffat. In their article (see references) they showed that illumination of the fusion kinase YF1 reduced net kinase activity by ∼1000-fold in vitro. They also showed that YF1 also controls gene expression in a light-dependent manner in vivo.
 
YF1 is a fusion protein of YtvA (''B subtilis'') and FixL (''B japonicum''). It contains the N-terminal region of YtvA (1-127) and C-terminal region of FixL (258-505), interconnected by a helical linker. This fusion protein is designed by Andreas Moglich and Keith Moffat. In their article (see references) they showed that illumination of the fusion kinase YF1 reduced net kinase activity by ∼1000-fold in vitro. They also showed that YF1 also controls gene expression in a light-dependent manner in vivo.
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'''References'''
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Moglich A, Ayers RA and Moffat K. (2009) Design and Signaling Mechanism of Light-Regulated Histidine Kinases. J. Mol. Bio. 385, 5, 1433-1444.
  
  

Revision as of 10:36, 21 September 2011

YF1 blue-light sensor protein

YF1 is a fusion protein of YtvA (B subtilis) and FixL (B japonicum). It contains the N-terminal region of YtvA (1-127) and C-terminal region of FixL (258-505), interconnected by a helical linker. This fusion protein is designed by Andreas Moglich and Keith Moffat. In their article (see references) they showed that illumination of the fusion kinase YF1 reduced net kinase activity by ∼1000-fold in vitro. They also showed that YF1 also controls gene expression in a light-dependent manner in vivo.

References

Moglich A, Ayers RA and Moffat K. (2009) Design and Signaling Mechanism of Light-Regulated Histidine Kinases. J. Mol. Bio. 385, 5, 1433-1444.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 544
    Illegal NgoMIV site found at 616
    Illegal NgoMIV site found at 706
    Illegal NgoMIV site found at 724
    Illegal AgeI site found at 258
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 157