Difference between revisions of "Part:BBa K606035"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K606035 short</partinfo> | <partinfo>BBa_K606035 short</partinfo> | ||
− | GFP expression system | + | T7 RNA polymease controlled GFP expression system for B. subtilis and E. coli |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
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+ | This part is the concatenation of the promoter pT7 (I719004) with the RBS SpoVG (K143021), that is design for B. subtilis but that also works in E. coli. This part works very efficiently and have been caracterized in E. coli BL21 cells. | ||
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Revision as of 09:48, 21 September 2011
pT7 SpoVG GFP T7terminator.
T7 RNA polymease controlled GFP expression system for B. subtilis and E. coli
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 917
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 693