Difference between revisions of "Part:BBa K606035"

 
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<partinfo>BBa_K606035 short</partinfo>
 
<partinfo>BBa_K606035 short</partinfo>
  
GFP expression system in presence of T7 polymerase
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T7 RNA polymease controlled GFP expression system for B. subtilis and E. coli
  
 
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===Usage and Biology===
 
===Usage and Biology===
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This part is the concatenation of the promoter pT7 (I719004) with the RBS SpoVG (K143021), that is design for B. subtilis but that also works in E. coli. This part works very efficiently and have been caracterized in E. coli BL21 cells.
  
 
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Revision as of 09:48, 21 September 2011

pT7 SpoVG GFP T7terminator.

T7 RNA polymease controlled GFP expression system for B. subtilis and E. coli

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 917
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 693