Difference between revisions of "Part:BBa K568005:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | + | Red light sensor (<html><a href="https://parts.igem.org/Part:BBa_K568000">BBa_K568000</a></html>) added to intermediated part of optogenetic AND gate (<html><a href="https://parts.igem.org/Part:BBa_K568006">BBa_K568006</a></html>). | |
− | + | Next step is cloning of T7ptag (<html><a href="https://parts.igem.org Part:BBa_K228000">BBa_K228000</a></html> at 3' site of intermediate construct. | |
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===Source=== | ===Source=== |
Revision as of 22:10, 20 September 2011
Intermediate optogenetical AND-Gate without T7ptag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 644
Illegal XhoI site found at 1786 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 971
Illegal AgeI site found at 3817 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 3820
Design Notes
Red light sensor (BBa_K568000) added to intermediated part of optogenetic AND gate (BBa_K568006). Next step is cloning of T7ptag (BBa_K228000 at 3' site of intermediate construct.
Source
tbd