Difference between revisions of "Part:BBa K553000:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | the part was generated by | + | the part was generated by using the following primers: |
− | |||
− | |||
− | |||
'''TraR fw ''' | '''TraR fw ''' | ||
Line 17: | Line 14: | ||
5' GTTTCTTCGAATTCGCGGCCGCTTCTAGATCGACGACTGGCTGGACAAGC 3' | 5' GTTTCTTCGAATTCGCGGCCGCTTCTAGATCGACGACTGGCTGGACAAGC 3' | ||
− | + | '''TraR rev ''' | |
+ | |||
+ | 5' GTTTCTTCCTGCAGCGGCCGCTACTAGTATCAGATGAGTTTCCGCCGGATG 3' | ||
+ | |||
+ | |||
+ | pcr protocol: | ||
95° 5' | 10x (93° 30" | 56° 30" | 72° 40") | 23x ( (93° 30" | 65° 30" | 72° 40") | 72° 7' | 4° ∞ | 95° 5' | 10x (93° 30" | 56° 30" | 72° 40") | 23x ( (93° 30" | 65° 30" | 72° 40") | 72° 7' | 4° ∞ | ||
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===Source=== | ===Source=== | ||
− | The gene was extracted by pcr from Agrobacterium | + | The gene was extracted by pcr from ''Agrobacterium tumefaciens'' gDNA. |
− | http://www.ncbi.nlm.nih.gov/ | + | http://www.ncbi.nlm.nih.gov/gene/1224220 |
The sequencing revealed mutations in our strain that do not affect the part functionality. | The sequencing revealed mutations in our strain that do not affect the part functionality. | ||
===References=== | ===References=== | ||
+ | |||
+ | Piper KR, Beck von Bodman S, Far- rand SK. 1993. ''Conjugation factor of Agrobacterium tumefaciens regulates Ti plasmid transfer by autoinduction''. Nature 362:448–50 | ||
+ | |||
+ | Zhang L, Murphy PJ, Kerr A, Tate ME. 1993. ''Agrobacterium conjugation and gene regulation by N-acyl-L-homoserine lactones''. Nature 362:446–48 |
Latest revision as of 21:47, 20 September 2011
TraR trans-activator
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
the part was generated by using the following primers:
TraR fw
5' GTTTCTTCGAATTCGCGGCCGCTTCTAGATCGACGACTGGCTGGACAAGC 3'
TraR rev
5' GTTTCTTCCTGCAGCGGCCGCTACTAGTATCAGATGAGTTTCCGCCGGATG 3'
pcr protocol:
95° 5' | 10x (93° 30" | 56° 30" | 72° 40") | 23x ( (93° 30" | 65° 30" | 72° 40") | 72° 7' | 4° ∞
The part contains also the BBa_B0034 RBS and BBa_B0015 double terminator.
Source
The gene was extracted by pcr from Agrobacterium tumefaciens gDNA.
http://www.ncbi.nlm.nih.gov/gene/1224220
The sequencing revealed mutations in our strain that do not affect the part functionality.
References
Piper KR, Beck von Bodman S, Far- rand SK. 1993. Conjugation factor of Agrobacterium tumefaciens regulates Ti plasmid transfer by autoinduction. Nature 362:448–50
Zhang L, Murphy PJ, Kerr A, Tate ME. 1993. Agrobacterium conjugation and gene regulation by N-acyl-L-homoserine lactones. Nature 362:446–48