Difference between revisions of "Part:BBa K678051"

 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K678051 short</partinfo>
 
<partinfo>BBa_K678051 short</partinfo>
  
−
To be announced
+
pJEJAM3 is a plasmid intended for transient transfection of mammalian cells. pJEJAM3 consists of the strong constitutive cytomegalovirus (CMV) promoter, yellow fluorescent protein (YFP), the ''BGK'' polyA, the ''hygR'' marker cassette, an ori for propagation in ''E.coli'' and, ampR resistance cassette (Figure 1).
 +
 
 +
<br>
 +
<br>
 +
[[Image:Devices_14.png|none|500px|thumb|<b>DTU-Denmark-2 2011</b> Figure 1: Plasmid map of pJEJAM3, the figure is not drawn to scale.]]
 +
<br>
 +
<br>
 +
 
 +
 
 +
[[Image:14%2B16_gul.png|left|300px|thumb|<b>DTU-Denmark-2 2011</b> Figure 2: .]]
 +
[[Image:1_14_gul.png|right|300px|thumb|<b>DTU-Denmark-2 2011</b> Figure 3: .]]
 +
<br clear=all>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 21:14, 20 September 2011

pJEJAM3

pJEJAM3 is a plasmid intended for transient transfection of mammalian cells. pJEJAM3 consists of the strong constitutive cytomegalovirus (CMV) promoter, yellow fluorescent protein (YFP), the BGK polyA, the hygR marker cassette, an ori for propagation in E.coli and, ampR resistance cassette (Figure 1).



DTU-Denmark-2 2011 Figure 1: Plasmid map of pJEJAM3, the figure is not drawn to scale.




DTU-Denmark-2 2011 Figure 2: .
DTU-Denmark-2 2011 Figure 3: .


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1416
    Illegal EcoRI site found at 2294
    Illegal XbaI site found at 1459
    Illegal PstI site found at 874
    Illegal PstI site found at 1421
    Illegal PstI site found at 2384
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1416
    Illegal EcoRI site found at 2294
    Illegal PstI site found at 874
    Illegal PstI site found at 1421
    Illegal PstI site found at 2384
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1416
    Illegal EcoRI site found at 2294
    Illegal BglII site found at 1396
    Illegal BamHI site found at 1447
    Illegal XhoI site found at 1400
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1416
    Illegal EcoRI site found at 2294
    Illegal XbaI site found at 1459
    Illegal PstI site found at 874
    Illegal PstI site found at 1421
    Illegal PstI site found at 2384
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1416
    Illegal EcoRI site found at 2294
    Illegal XbaI site found at 1459
    Illegal PstI site found at 874
    Illegal PstI site found at 1421
    Illegal PstI site found at 2384
    Illegal NgoMIV site found at 3149
  • 1000
    COMPATIBLE WITH RFC[1000]