Difference between revisions of "Part:BBa K608101"
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| − | + | ==Usage and Biology== | |
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| − | when we amplified CcaR from the whole Synechocystis genome via PCR, <br/> the primer had an overhang which also has a Ribosome | + | when we amplified CcaR from the whole Synechocystis genome via PCR, <br/> the primer had an overhang which also has a Ribosome Binding Site.<br/> |
If you want to use it, you can clone it behind a promotor of choice. | If you want to use it, you can clone it behind a promotor of choice. | ||
Revision as of 20:46, 20 September 2011
CcaR, green light response regulator
CcaR is the response regulator to the green light receptor CcaS.
It belongs to the family of OmpR regulator class. CcaR consists of an N-terminal receiver domain that can be
phosphorylated by CcaS and a C-terminal DNA-binding domain that binds directly to the promoter region of cpcG2.
Usage and Biology
when we amplified CcaR from the whole Synechocystis genome via PCR,
the primer had an overhang which also has a Ribosome Binding Site.
If you want to use it, you can clone it behind a promotor of choice.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 380
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]