Difference between revisions of "Part:BBa K590017"

 
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__NOTOC__
 
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<partinfo>BBa_K590017 short</partinfo>
 
<partinfo>BBa_K590017 short</partinfo>
  
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First part of full assembly
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This part is made by [http://2011.igem.org/Team:Washington UW iGEM Team 2011]. It includes 6 genes that were found essential for magnetosome formation in ''Magnetospirillum magneticum'' strain AMB-1 on a [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590014 pGA3k3 plasmid backbone].
  
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
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This is the product made by Gibson Cloning method instead of Standard Biobrick method. It belongs to the [http://2011.igem.org/Team:Washington/Magnetosomes/Magnet_Toolkit Magnetosome Toolkit].
  
 
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Revision as of 20:37, 20 September 2011

mamHIEJKL in vector pGA3K3

This part is made by [http://2011.igem.org/Team:Washington UW iGEM Team 2011]. It includes 6 genes that were found essential for magnetosome formation in Magnetospirillum magneticum strain AMB-1 on a pGA3k3 plasmid backbone.

Usage and Biology

This is the product made by Gibson Cloning method instead of Standard Biobrick method. It belongs to the [http://2011.igem.org/Team:Washington/Magnetosomes/Magnet_Toolkit Magnetosome Toolkit].

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 5175
    Illegal PstI site found at 8836
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 5175
    Illegal PstI site found at 8836
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 8540
    Illegal XhoI site found at 89
    Illegal XhoI site found at 6375
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 5175
    Illegal PstI site found at 8836
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 5175
    Illegal PstI site found at 8836
    Illegal NgoMIV site found at 238
    Illegal NgoMIV site found at 1254
    Illegal NgoMIV site found at 2589
    Illegal NgoMIV site found at 3920
    Illegal NgoMIV site found at 6754
    Illegal NgoMIV site found at 8562
    Illegal NgoMIV site found at 8929
    Illegal AgeI site found at 5849
    Illegal AgeI site found at 6264
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 651
    Illegal BsaI site found at 1986
    Illegal BsaI site found at 3317
    Illegal BsaI.rc site found at 6947
    Illegal SapI site found at 825
    Illegal SapI site found at 2160
    Illegal SapI site found at 3491
    Illegal SapI.rc site found at 9208
    Illegal SapI.rc site found at 9529