Difference between revisions of "Part:BBa K678049"
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<partinfo>BBa_K678049 short</partinfo>
 
<partinfo>BBa_K678049 short</partinfo>
  
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JEJAM1 is a plasmid intended for transient transfection of mammalian cells. JEJAM1 consist of the strong constitutive promoter, ''CMV'', the green fluorescence protein (GFP), the ''BGK'' polyA terminator and the ''hygR'' marker cassette used for selection, an ori for propagation in ''E.coli'' and and ampR resistance cassette (Figure below).
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pJEJAM1 is a plasmid intended for transient transfection of mammalian cells. pJEJAM1 consists of the strong constitutive cytomegalovirus (CMV) promoter, the green fluorescence protein (GFP), the ''BGK'' polyA and the ''hygR'' marker cassette used for selection, an ori for propagation in ''E.coli'' and and ampR resistance cassette (Figure below).
  
  

Revision as of 20:20, 20 September 2011

pJEJAM1

pJEJAM1 is a plasmid intended for transient transfection of mammalian cells. pJEJAM1 consists of the strong constitutive cytomegalovirus (CMV) promoter, the green fluorescence protein (GFP), the BGK polyA and the hygR marker cassette used for selection, an ori for propagation in E.coli and and ampR resistance cassette (Figure below).


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2216
    Illegal PstI site found at 2306
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2216
    Illegal PstI site found at 2306
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2216
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2216
    Illegal PstI site found at 2306
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2216
    Illegal PstI site found at 2306
    Illegal NgoMIV site found at 3071
  • 1000
    COMPATIBLE WITH RFC[1000]