Difference between revisions of "Part:BBa K525121:Design"
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+ | Hansmeier N, Bartels FW, Ros R, Anselmetti D, Tauch A, Pühler A, Kalinowski J (2004) Classification of hyper-variable Corynebacterium glutamicum surface-layer proteins by sequence analyses and atomic force microscopy [http://www.sciencedirect.com/science/article/pii/S016816560400241X J Biotechnol. 26;112(1-2):177-93.] |
Revision as of 19:53, 20 September 2011
S-layer cspB from Corynebacterium glutamicum with TAT-Sequence and lipid anchor, PT7 and RBS
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1421
Illegal XhoI site found at 248
Illegal XhoI site found at 866 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1400
Illegal SapI site found at 647
Illegal SapI site found at 859
Illegal SapI site found at 1407
Design Notes
PCR
Source
- PCR on the Corynebacterium glutamicum (ATCC 14067) DNA cloned into BioBrick Vectors
- Primers with BioBrick prefix, pT7-Promoter and RBS in the fwd primer. Suffix in the rev primer.
- fwd: GAATTCGCGGCCGCTTCTAGAGTAATACGACTCACTATAGGGAAAGAGGAGAAAATGTTTAACAACCGTATCCGCAC
- rev: CTGCAGCGGCCGCTACTAGTATTAGAACTTAACGATACCGGAGAGG
- Primers with BioBrick prefix, pT7-Promoter and RBS in the fwd primer. Suffix in the rev primer.
- [http://www.ncbi.nlm.nih.gov/nuccore/AY842007.1 GenBank: AY842007.1]
References
Hansmeier N, Bartels FW, Ros R, Anselmetti D, Tauch A, Pühler A, Kalinowski J (2004) Classification of hyper-variable Corynebacterium glutamicum surface-layer proteins by sequence analyses and atomic force microscopy [http://www.sciencedirect.com/science/article/pii/S016816560400241X J Biotechnol. 26;112(1-2):177-93.]