Difference between revisions of "Part:BBa K678060"

 
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<partinfo>BBa_K678060 short</partinfo>
 
<partinfo>BBa_K678060 short</partinfo>
  
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pJEJAM15 is a plasmid that after digestion with NotI is intended to be transformed into ''Aspergillus nidulans'' and integrated into the genome by non-homologous end joining. The plasmid consists the strong constitutive ''gpdA'' promoter , mRFP with a nucleosomal targeting sequence at the C-terminus, the ''trpC'' terminator, a ''pyrG'' marker cassette for selection, an ori for propagation in ''E. coli'', and an ampR resistance cassette (Figure 1).
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[[Image:Device_25.png|none|500px|thumb|<b>DTU-Denmark-2 2011</b> Figure 1: Plasmid map of pJEJAM1, the figure is not drawn to scale.]]
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consists of RFP with a target signal to the nucleus which can explain the spots compared to the results from device and the spores with a single nuclei contains a lot of RFP signal. We cannot conclude that the signal is accumulated in the nucleus since they are not dyed, though can it be concluded that the RFP signal is target a specific place and accumulated somewhere.
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[[Image:25_dic_t_1_png.png|left|300px|thumb|<b>DTU-Denmark-2 2011</b> Figure 2: ''Aspergillus nidulans'' with device DIC light.]]
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[[Image:NyRFP.jpg|right|300px|thumb|<b>DTU-Denmark-2 2011</b> Figure 2: ''Aspergillus nidulans'' with device DIC light.]]
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[[Image:25.14_front_rettet.jpg|left|300px|thumb|<b>DTU-Denmark-2 2011</b> Figure 2: ''Aspergillus nidulans'' Front]]
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[[Image:25.14_back_rettet.jpg|right|300px|thumb|<b>DTU-Denmark-2 2011</b> Figure 2: ''Aspergillus nidulans'' Back]]
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 13:22, 20 September 2011

pJEJAM12

pJEJAM15 is a plasmid that after digestion with NotI is intended to be transformed into Aspergillus nidulans and integrated into the genome by non-homologous end joining. The plasmid consists the strong constitutive gpdA promoter , mRFP with a nucleosomal targeting sequence at the C-terminus, the trpC terminator, a pyrG marker cassette for selection, an ori for propagation in E. coli, and an ampR resistance cassette (Figure 1).

DTU-Denmark-2 2011 Figure 1: Plasmid map of pJEJAM1, the figure is not drawn to scale.



consists of RFP with a target signal to the nucleus which can explain the spots compared to the results from device and the spores with a single nuclei contains a lot of RFP signal. We cannot conclude that the signal is accumulated in the nucleus since they are not dyed, though can it be concluded that the RFP signal is target a specific place and accumulated somewhere.

DTU-Denmark-2 2011 Figure 2: Aspergillus nidulans with device DIC light.
DTU-Denmark-2 2011 Figure 2: Aspergillus nidulans with device DIC light.


DTU-Denmark-2 2011 Figure 2: Aspergillus nidulans Front
DTU-Denmark-2 2011 Figure 2: Aspergillus nidulans Back


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 3768
    Illegal XbaI site found at 5324
    Illegal XbaI site found at 8068
    Illegal PstI site found at 140
    Illegal PstI site found at 4609
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 910
    Illegal PstI site found at 140
    Illegal PstI site found at 4609
    Illegal NotI site found at 5281
    Illegal NotI site found at 8107
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 144
    Illegal BamHI site found at 3049
    Illegal BamHI site found at 4347
    Illegal XhoI site found at 700
    Illegal XhoI site found at 1082
    Illegal XhoI site found at 4207
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 3768
    Illegal XbaI site found at 5324
    Illegal XbaI site found at 8068
    Illegal PstI site found at 140
    Illegal PstI site found at 4609
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 3768
    Illegal XbaI site found at 5324
    Illegal XbaI site found at 8068
    Illegal PstI site found at 140
    Illegal PstI site found at 4609
    Illegal NgoMIV site found at 3685
    Illegal AgeI site found at 1289
    Illegal AgeI site found at 1437
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4400
    Illegal BsaI.rc site found at 1116
    Illegal BsaI.rc site found at 3399
    Illegal SapI site found at 322