Difference between revisions of "Part:BBa K510021"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K510021 short</partinfo> | <partinfo>BBa_K510021 short</partinfo> | ||
− | The mini-Tn7-Gm-Lux artificial transposon has been constructed based on pUC18R6KT-miniTn7-Gm (BBa_K510012) by inserting a pBad/Lux operon (BBa_K325909) in its BioBrick cloning site (BCS). This plasmid can be used for visualization purposes or to brand a strain because of its insertion in the genome of the working organism and split the antibiotic resistance cassette with the temporary expression of Flp recombinase. | + | |
+ | The mini-Tn7-Gm-Lux artificial transposon has been constructed based on pUC18R6KT-miniTn7-Gm ([https://parts.igem.org/Part:BBa_K510012 BBa_K510012]) by inserting a pBad/Lux operon ([https://parts.igem.org/Part:BBa_K325909 BBa_K325909]) in its BioBrick cloning site (BCS). This plasmid can be used for visualization purposes or to brand a strain because of its insertion in the genome of the working organism and split the antibiotic resistance cassette with the temporary expression of Flp recombinase. | ||
+ | |||
+ | [[Image:Mini-Tn7-Gm-K325909.png|700px|center]] | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 22:04, 19 September 2011
pUC18R6KT-miniTn7BB-Gm-Lux
The mini-Tn7-Gm-Lux artificial transposon has been constructed based on pUC18R6KT-miniTn7-Gm (BBa_K510012) by inserting a pBad/Lux operon (BBa_K325909) in its BioBrick cloning site (BCS). This plasmid can be used for visualization purposes or to brand a strain because of its insertion in the genome of the working organism and split the antibiotic resistance cassette with the temporary expression of Flp recombinase.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 4528
Illegal suffix found in sequence at 1 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 4528
Illegal NheI site found at 5762
Illegal NheI site found at 8728
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 4534 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 4528
Illegal BglII site found at 3212
Illegal BglII site found at 3483
Illegal BglII site found at 3769
Illegal BglII site found at 7726
Illegal BamHI site found at 5701
Illegal XhoI site found at 8556 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 4528
Illegal suffix found in sequence at 2 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 4528
Illegal XbaI site found at 4543
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal AgeI site found at 5536 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 10115
Illegal BsaI.rc site found at 1854
Illegal BsaI.rc site found at 7124
Illegal SapI site found at 518
Illegal SapI site found at 5518
Illegal SapI.rc site found at 10440