Difference between revisions of "Part:BBa K510018"

 
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<partinfo>BBa_K510018 short</partinfo>
 
<partinfo>BBa_K510018 short</partinfo>
  
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The mini-Tn7-Gm-RBS+RFP artificial transposon has been constructed based on pUC18R6KT-miniTn7-Gm (BBa_K510012) by inserting a lacZ-alfa+GFP-AAV cassette (BBa_I732094) in its BioBrick cloning site (BCS). This plasmid can be used for promoter characterization purposes inserting the promoter using the prefix restriction sites. Also the characterization can be performed in single copy by integrating the device in the genome of the working organism.
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The mini-Tn7-Gm-RBS+RFP artificial transposon has been constructed based on pUC18R6KT-miniTn7-Gm ([https://parts.igem.org/Part:BBa_K510012 BBa_K510012]) by inserting a lacZ-alfa+GFP-AAV cassette ([https://parts.igem.org/Part:BBa_I732094 BBa_I732094]) in its BioBrick cloning site (BCS). This plasmid can be used for promoter characterization purposes inserting the promoter using the prefix restriction sites. Also the characterization can be performed in single copy by integrating the device in the genome of the working organism.
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[[Image:Mini-Tn7-Gm-I732094.png|700px|center]]
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 21:43, 19 September 2011

pUC18R6KT-miniTn7BB-Gm-lacZ+GFP


The mini-Tn7-Gm-RBS+RFP artificial transposon has been constructed based on pUC18R6KT-miniTn7-Gm (BBa_K510012) by inserting a lacZ-alfa+GFP-AAV cassette (BBa_I732094) in its BioBrick cloning site (BCS). This plasmid can be used for promoter characterization purposes inserting the promoter using the prefix restriction sites. Also the characterization can be performed in single copy by integrating the device in the genome of the working organism.

Mini-Tn7-Gm-I732094.png

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 4528
    Illegal suffix found in sequence at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4528
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 4534
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4528
    Illegal BglII site found at 3212
    Illegal BglII site found at 3483
    Illegal BglII site found at 3769
    Illegal BglII site found at 4560
    Illegal BamHI site found at 4569
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 4528
    Illegal suffix found in sequence at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 4528
    Illegal XbaI site found at 4543
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1854
    Illegal BsaI.rc site found at 5506
    Illegal SapI site found at 518