Difference between revisions of "Part:BBa K596001"
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<partinfo>BBa_K596001 short</partinfo> | <partinfo>BBa_K596001 short</partinfo> | ||
Nitroreductase is a protein and it removes nitro functional group make it less toxic molecules; such as from TNT to monoaminodinitrotoluene(ADNT), 2,4-dinitrotoluene(DNT).Enterobacter cloacae oxygen-insensitive NAD(P)H nitroreductase (nfsI) gene is our basic gene. | Nitroreductase is a protein and it removes nitro functional group make it less toxic molecules; such as from TNT to monoaminodinitrotoluene(ADNT), 2,4-dinitrotoluene(DNT).Enterobacter cloacae oxygen-insensitive NAD(P)H nitroreductase (nfsI) gene is our basic gene. | ||
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+ | Author: C.Bryant at all. | ||
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+ | Reference: | ||
+ | 1. Bryant C, Hubbard L, McElroy WD. Cloning, nucleotide sequence, and expression of the nitroreductase gene from Enterobacter cloacae. The Journal of biological chemistry. 1991;266(7):4126-30. Available at: http://www.ncbi.nlm.nih.gov/pubmed/1999406. | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Latest revision as of 20:57, 19 September 2011
Nitroreductase optimized for C.reinhardtii
Nitroreductase is a protein and it removes nitro functional group make it less toxic molecules; such as from TNT to monoaminodinitrotoluene(ADNT), 2,4-dinitrotoluene(DNT).Enterobacter cloacae oxygen-insensitive NAD(P)H nitroreductase (nfsI) gene is our basic gene.
Author: C.Bryant at all.
Reference: 1. Bryant C, Hubbard L, McElroy WD. Cloning, nucleotide sequence, and expression of the nitroreductase gene from Enterobacter cloacae. The Journal of biological chemistry. 1991;266(7):4126-30. Available at: http://www.ncbi.nlm.nih.gov/pubmed/1999406.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 661
Illegal XhoI site found at 1 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 199
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 618