Difference between revisions of "Part:BBa K638301"

Revision as of 19:49, 19 September 2011

Arabinose inducible Reflectin A1-sfGFP fusion generator

E.coli transformed with this construct will produce a superfolder Green fluorescent Protein (sfGFP) fused variant of reflectin A1 under the control of pBAD, an arabinose inducible protein. There is currently (at the time of writing) no good assay for reflectin, in vivo. This construct allows reflectin to be localised within the cell. This part has been used to view reflectin containing inclusion bodies in E.coli and the sfGFP retained its fluorescence despite the fusion.

Sequence and Features