Difference between revisions of "Part:BBa K584015"
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<partinfo>BBa_K584015 short</partinfo> | <partinfo>BBa_K584015 short</partinfo> | ||
| − | We performed a '''PCR''' to get the '''CeaB''' part of the '''K131009 biobrick'''. The primer design | + | We performed a '''PCR''' to get the '''CeaB''' part of the '''K131009 biobrick'''. The primer design for this was such that it already contained the ribolock resulting in ‘Ribolock + ceaB’ as the final product. This is fused with the terminator B0015 to get ‘Ribolock + ceaB + B0015’. This was finally fused with the Lambda cI and LuxR regulated hybrid promotor to get this biobrick, ‘Lambda_cI and LuxR_regulated hybrid promotor’ + Ribolock + ceaB + B0015. |
| − | + | The primer sequence we used in the PCR reaction is - | |
ceaB-FW: CCGGAATTCGCGGCCGCTTCTAGAGAACTAGAATCACCTCTTGCTTTTGGGTAAGACGAAGAGGAGATACTAGATGAGCGGTGGCGATGGACGC | ceaB-FW: CCGGAATTCGCGGCCGCTTCTAGAGAACTAGAATCACCTCTTGCTTTTGGGTAAGACGAAGAGGAGATACTAGATGAGCGGTGGCGATGGACGC | ||
Revision as of 09:52, 19 September 2011
Lambda cI and LuxR regulated hybrid promoter + Ribolock-CeaB + Terminator
We performed a PCR to get the CeaB part of the K131009 biobrick. The primer design for this was such that it already contained the ribolock resulting in ‘Ribolock + ceaB’ as the final product. This is fused with the terminator B0015 to get ‘Ribolock + ceaB + B0015’. This was finally fused with the Lambda cI and LuxR regulated hybrid promotor to get this biobrick, ‘Lambda_cI and LuxR_regulated hybrid promotor’ + Ribolock + ceaB + B0015.
The primer sequence we used in the PCR reaction is -
ceaB-FW: CCGGAATTCGCGGCCGCTTCTAGAGAACTAGAATCACCTCTTGCTTTTGGGTAAGACGAAGAGGAGATACTAGATGAGCGGTGGCGATGGACGC
ceaB-RV: AAAACTGCAGCGGCCGCTACTAGTCTCTAGTATTACTTACCCCGATGAATATCAATAT
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]