Difference between revisions of "Part:BBa K590015"
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mamK was previously reported for its importance proper magnetosome chain organization in magnetic bacteria; it is bacterial actin-like cytoskeleton protein required for proper alignment of the magnetosomes in a chain. | mamK was previously reported for its importance proper magnetosome chain organization in magnetic bacteria; it is bacterial actin-like cytoskeleton protein required for proper alignment of the magnetosomes in a chain. | ||
| − | This part was transformed into ''E.coli'' and | + | This part was transformed into ''E.coli'' and fluorescent filament structures were observed: |
| − | + | [[Image: MamK_sfGFP_3k3-1_uwigem2011part.jpg|700px|center]] | |
| − | + | ||
Revision as of 05:54, 19 September 2011
sfGFP_mamK_pGA1C3
This part was made by [http://2011.igem.org/Team:Washington UW iGEM Team 2011] and was contributed to the [http://2011.igem.org/Team:Washington/Magnetosomes/Results Magnetosome Toolkit].
Usage and Biology
This part consists of mamK gene from Magnetospirillum magneticum strain AMB-1, sfGFP in the backbone of pGA3k3. It was made by Gibson Cloning.
mamK was previously reported for its importance proper magnetosome chain organization in magnetic bacteria; it is bacterial actin-like cytoskeleton protein required for proper alignment of the magnetosomes in a chain.
This part was transformed into E.coli and fluorescent filament structures were observed:
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1430
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 1430
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1134
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1430
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1430
Illegal NgoMIV site found at 1156
Illegal NgoMIV site found at 1523
Illegal NgoMIV site found at 1670 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 80
Illegal SapI.rc site found at 1802