Difference between revisions of "Part:BBa K528007"
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===Measurements=== | ===Measurements=== | ||
| − | All our | + | Using this construct we have determined that strength of BBa_B0031 with YFP is 83,42% relative to BBa_B0034 with YFP. |
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| + | All our YFP measurement results are summarized on the chart below: | ||
https://static.igem.org/mediawiki/parts/9/90/Wyk7.png | https://static.igem.org/mediawiki/parts/9/90/Wyk7.png | ||
Latest revision as of 21:57, 18 September 2011
RBS measurement device J23100 B0031 YFP
This device was used to determine BBa_B0031 strength relative to BBa_B0034.
Construct design
The device consists of BBa_K299501 expression vector J23100+B0031. E0030 YFP was used as reporter protein.
Authors
Clonning:
- Paweł Urbański
- Elżbieta Jankowska
- Krzysztof Szczepaniak
- Dorota Kaja Sabat
The lab work was supervised by:
- Michał Lower
- Ania Olchowik
Measurements
Using this construct we have determined that strength of BBa_B0031 with YFP is 83,42% relative to BBa_B0034 with YFP.
All our YFP measurement results are summarized on the chart below:
For more info please look at Team Warsaw 2011 [http://2011.igem.org/Team:Warsaw/RBSmeasurement].
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]