Difference between revisions of "Part:BBa K528005"

 
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__NOTOC__
 
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<partinfo>BBa_K528005 short</partinfo>
 
<partinfo>BBa_K528005 short</partinfo>
  
 
This device was used to determine BBa_B0033 strength relative to BBa_B0034.
 
This device was used to determine BBa_B0033 strength relative to BBa_B0034.
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 +
===Construct design===
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The device consists of BBa_K299503 expression vector J23100+B0033. E1010 RFP was used as reporter protein.
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 +
===Authors===
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Clonning:
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*Paweł Urbański
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*Elżbieta Jankowska
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*Krzysztof Szczepaniak
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*Dorota Kaja Sabat
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 +
The lab work was supervised by:
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*Michał Lower
 +
*Ania Olchowik
 +
 +
===Measurements===
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Using this construct we have determined that strength of BBa_B0033 with RFP is 13,73% relative to BBa_B0034 with RFP.
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 +
All our RFP measurement results are summarized on the chart below:
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https://static.igem.org/mediawiki/parts/0/0e/Wyk6.png 
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 +
For more info please look at Team Warsaw 2011 [http://2011.igem.org/Team:Warsaw/RBSmeasurement].
  
 
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Latest revision as of 21:55, 18 September 2011

RBS measurement device J23100 B0033 RFP

This device was used to determine BBa_B0033 strength relative to BBa_B0034.

Construct design

The device consists of BBa_K299503 expression vector J23100+B0033. E1010 RFP was used as reporter protein.

Authors

Clonning:

  • Paweł Urbański
  • Elżbieta Jankowska
  • Krzysztof Szczepaniak
  • Dorota Kaja Sabat

The lab work was supervised by:

  • Michał Lower
  • Ania Olchowik

Measurements

Using this construct we have determined that strength of BBa_B0033 with RFP is 13,73% relative to BBa_B0034 with RFP.

All our RFP measurement results are summarized on the chart below:

Wyk6.png

For more info please look at Team Warsaw 2011 [http://2011.igem.org/Team:Warsaw/RBSmeasurement].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 615
    Illegal AgeI site found at 727
  • 1000
    COMPATIBLE WITH RFC[1000]