Difference between revisions of "Part:BBa K528003"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K528003 short</partinfo> | <partinfo>BBa_K528003 short</partinfo> | ||
| − | This device was used to determine BBa_B0031 strength relative to BBa_B0034. | + | This device was used to determine BBa_B0031 strength relative to BBa_B0034. |
| + | |||
| + | ===Construct design=== | ||
| + | The device consists of BBa_K299501 expression vector J23100+B0031. E1010 RFP was used as reporter protein. | ||
| + | |||
| + | ===Authors=== | ||
| + | Clonning: | ||
| + | *Paweł Urbański | ||
| + | *Elżbieta Jankowska | ||
| + | *Krzysztof Szczepaniak | ||
| + | *Dorota Kaja Sabat | ||
| + | |||
| + | The lab work was supervised by: | ||
| + | *Michał Lower | ||
| + | *Ania Olchowik | ||
| + | |||
| + | ===Measurements=== | ||
| + | |||
| + | Using this construct we have determined that strength of BBa_B0031 with RFP is 8,29% relative to BBa_B0034 with RFP. | ||
| + | |||
| + | All our RFP measurement results are summarized on the chart below: | ||
| + | |||
| + | https://static.igem.org/mediawiki/parts/0/0e/Wyk6.png | ||
| + | |||
| + | For more info please look at Team Warsaw 2011 [http://2011.igem.org/Team:Warsaw/RBSmeasurement]. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 21:51, 18 September 2011
RBS measurement device J23100 B0031 RFP
This device was used to determine BBa_B0031 strength relative to BBa_B0034.
Construct design
The device consists of BBa_K299501 expression vector J23100+B0031. E1010 RFP was used as reporter protein.
Authors
Clonning:
- Paweł Urbański
- Elżbieta Jankowska
- Krzysztof Szczepaniak
- Dorota Kaja Sabat
The lab work was supervised by:
- Michał Lower
- Ania Olchowik
Measurements
Using this construct we have determined that strength of BBa_B0031 with RFP is 8,29% relative to BBa_B0034 with RFP.
All our RFP measurement results are summarized on the chart below:
For more info please look at Team Warsaw 2011 [http://2011.igem.org/Team:Warsaw/RBSmeasurement].
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 618
Illegal AgeI site found at 730 - 1000COMPATIBLE WITH RFC[1000]