Difference between revisions of "Part:BBa K523009"
| (3 intermediate revisions by the same user not shown) | |||
| Line 2: | Line 2: | ||
<partinfo>BBa_K523009 short</partinfo> | <partinfo>BBa_K523009 short</partinfo> | ||
| − | This is a modified version of <partinfo>BBa_K118022</partinfo> for use with the BioSandwich protocol of Edinburgh 2011. | + | This is a modified version of <partinfo>BBa_K118022</partinfo> for use with the BioSandwich protocol (<nowiki>RFC 81</nowiki>) of Edinburgh 2011. |
| − | BioSandwich assembly uses an in-frame BglII site (formed by the last 2 bases of the RFC10 prefix and the first 4 bases at the start of this BioBrick) and an in-frame SpeI site (present in the RFC10 suffix). Because the SpeI site is out of frame, two bases ("TC") have been added at the end. When combined with the T at the start of the RFC10 suffix, they code for a serine residue and correct the frameshift. | + | When used to make fusion proteins, BioSandwich assembly uses an in-frame BglII site (formed by the last 2 bases of the RFC10 prefix and the first 4 bases at the start of this BioBrick) and an in-frame SpeI site (present in the RFC10 suffix). Because the SpeI site is out of frame, two bases ("TC") have been added at the end. When combined with the T at the start of the RFC10 suffix, they code for a serine residue and correct the frameshift. |
The signal peptide of the exoglucanase (amino acids 1 - 42) has been removed, since this part is intended for fusion to INP or other display proteins. | The signal peptide of the exoglucanase (amino acids 1 - 42) has been removed, since this part is intended for fusion to INP or other display proteins. | ||
| − | A non-synonymous change (see figure) was introduced by PCR. | + | A non-synonymous change (see figure) was accidentally introduced, probably by PCR. An aspartic acid residue has become a glycine. |
===Usage and Biology=== | ===Usage and Biology=== | ||
This is an exoglucanase from ''Cellulomonas fimi''. It degrades cellulose by chewing at the end of a cellulose chain to produce cellobiose sugar. | This is an exoglucanase from ''Cellulomonas fimi''. It degrades cellulose by chewing at the end of a cellulose chain to produce cellobiose sugar. | ||
| + | |||
<!-- --> | <!-- --> | ||
| − | <span class='h3bb'>Sequence and Features</span> | + | <span class='h3bb'>'''Sequence and Features'''</span> |
<partinfo>BBa_K523009 SequenceAndFeatures</partinfo> | <partinfo>BBa_K523009 SequenceAndFeatures</partinfo> | ||
Latest revision as of 20:18, 18 September 2011
Exoglucanase for BioSandwich
This is a modified version of BBa_K118022 for use with the BioSandwich protocol (RFC 81) of Edinburgh 2011.
When used to make fusion proteins, BioSandwich assembly uses an in-frame BglII site (formed by the last 2 bases of the RFC10 prefix and the first 4 bases at the start of this BioBrick) and an in-frame SpeI site (present in the RFC10 suffix). Because the SpeI site is out of frame, two bases ("TC") have been added at the end. When combined with the T at the start of the RFC10 suffix, they code for a serine residue and correct the frameshift.
The signal peptide of the exoglucanase (amino acids 1 - 42) has been removed, since this part is intended for fusion to INP or other display proteins.
A non-synonymous change (see figure) was accidentally introduced, probably by PCR. An aspartic acid residue has become a glycine.
Usage and Biology
This is an exoglucanase from Cellulomonas fimi. It degrades cellulose by chewing at the end of a cellulose chain to produce cellobiose sugar.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 405
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 38
Illegal NgoMIV site found at 411
Illegal NgoMIV site found at 913 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 458
Illegal SapI.rc site found at 541