Difference between revisions of "Part:BBa K523009"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K523009 short</partinfo>
 
<partinfo>BBa_K523009 short</partinfo>
  
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This is a modified version of <partinfo>BBa_K118022</partinfo> for use with the BioSandwich protocol of Edinburgh 2011.
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This is a modified version of <partinfo>BBa_K118022</partinfo> for use with the BioSandwich protocol (<nowiki>RFC 81</nowiki>) of Edinburgh 2011.
  
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BioSandwich assembly uses an in-frame BglII site (formed by the last 2 bases of the RFC10 prefix and the first 4 bases at the start of this BioBrick) and an in-frame SpeI site (present in the RFC10 suffix). Because the SpeI site is out of frame, two extra "G" bases have been added at the end. When combined with the T at the start of the RFC10 suffix, they code for a glycine residue and correct the frameshift.  
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When used to make fusion proteins, BioSandwich assembly uses an in-frame BglII site (formed by the last 2 bases of the RFC10 prefix and the first 4 bases at the start of this BioBrick) and an in-frame SpeI site (present in the RFC10 suffix). Because the SpeI site is out of frame, two bases ("TC") have been added at the end. When combined with the T at the start of the RFC10 suffix, they code for a serine residue and correct the frameshift.
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The signal peptide of the exoglucanase (amino acids 1 - 42) has been removed, since this part is intended for fusion to INP or other display proteins.
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A non-synonymous change (see figure) was accidentally introduced, probably by PCR. An aspartic acid residue has become a glycine.
  
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
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This is an exoglucanase from ''Cellulomonas fimi''. It degrades cellulose by chewing at the end of a cellulose chain to produce cellobiose sugar.
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<span class='h3bb'>Sequence and Features</span>
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<span class='h3bb'>'''Sequence and Features'''</span>
 
<partinfo>BBa_K523009 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K523009 SequenceAndFeatures</partinfo>
  

Latest revision as of 20:18, 18 September 2011

Exoglucanase for BioSandwich

This is a modified version of BBa_K118022 for use with the BioSandwich protocol (RFC 81) of Edinburgh 2011.

When used to make fusion proteins, BioSandwich assembly uses an in-frame BglII site (formed by the last 2 bases of the RFC10 prefix and the first 4 bases at the start of this BioBrick) and an in-frame SpeI site (present in the RFC10 suffix). Because the SpeI site is out of frame, two bases ("TC") have been added at the end. When combined with the T at the start of the RFC10 suffix, they code for a serine residue and correct the frameshift.

The signal peptide of the exoglucanase (amino acids 1 - 42) has been removed, since this part is intended for fusion to INP or other display proteins.

A non-synonymous change (see figure) was accidentally introduced, probably by PCR. An aspartic acid residue has become a glycine.

Usage and Biology

This is an exoglucanase from Cellulomonas fimi. It degrades cellulose by chewing at the end of a cellulose chain to produce cellobiose sugar.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 405
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 38
    Illegal NgoMIV site found at 411
    Illegal NgoMIV site found at 913
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 458
    Illegal SapI.rc site found at 541