Difference between revisions of "Part:BBa K523003"

Revision as of 20:14, 18 September 2011

malS for BioSandwich

This is a version of BBa_K523001 but with no RBS and no stop codon.

There are four extra bases at the end which (together with the standard RFC10 prefix) generate a BglII site. There are two extra bases at the end which, when combined with the T at the start of the standard RFC10 suffix, code for a glycine. The effect of both of these features is to provide an in-frame BglII site at the start, and an in-frame SpeI site at the end (from the standard suffix). These features are necessary for producing fusion proteins with Edinburgh 2011's BioSandwich assembly protocol (RFC 81).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1204
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 340
Illegal AgeI site found at 1505
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 This is a version of <partinfo>BBa_K523001</partinfo> but with no RBS and no stop codon.
-There are four extra bases at the end which (together with the standard RFC10 prefix) generate a BglII site.
+There are four extra bases at the end which (together with the standard RFC10 prefix) generate a BglII site. There are two extra bases at the end which, when combined with the T at the start of the standard RFC10 suffix, code for a glycine. The effect of both of these features is to provide an in-frame BglII site at the start, and an in-frame SpeI site at the end (from the standard suffix). These features are necessary for producing fusion proteins with Edinburgh 2011's BioSandwich assembly protocol (<nowiki>RFC 81</nowiki>).
-There are two extra bases at the end which, when combined with the T at the start of the standard RFC10 suffix, code for a glycine.
-The effect of both of these features is to provide an in-frame BglII site at the start, and an in-frame SpeI site at the end (from the standard suffix). These features are necessary for producing fusion proteins with Edinburgh 2011's BioSandwich assembly protocol (RFC 81).
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