Difference between revisions of "Part:BBa K510013:Design"
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+ | Kyoung-Hee Choi, Jared B Gaynor, Kimberly G White, Carolina Lopez, Catharine M Bosio, RoxAnn R Karkhoff-Schweizer & Herbert P Schweizer (2005). A Tn7-based broad-range bacterial cloning and expression system. Nature Methods, vol.2 NO.6, June 2005, 443. |
Revision as of 19:07, 18 September 2011
pUC18R6KT-miniTn7BB-Cm
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 4640
Illegal suffix found in sequence at 1 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 4640
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 4646 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 4640
Illegal BglII site found at 3212
Illegal BglII site found at 3483
Illegal XhoI site found at 3569 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 4640
Illegal suffix found in sequence at 2 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 4640
Illegal XbaI site found at 4655
Illegal SpeI site found at 2
Illegal PstI site found at 16 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1854
Illegal SapI site found at 518
Design Notes
Source
The pUC18R6KT-mini-Tn7-Cm is a derivative of pUC18R6KT-mini-Tn7-Gm (BBa_K510012) by replacing the gentamycin resistance cassette by a chloramphenicol resistance cassette amplified from pSB1C3 with these primers: tatGCATGCCCATGGaacttggtctgacagctcgag and tatGCATGCCCATGGtcgggcacgtaagaggttcc.
References
Kyoung-Hee Choi, Jared B Gaynor, Kimberly G White, Carolina Lopez, Catharine M Bosio, RoxAnn R Karkhoff-Schweizer & Herbert P Schweizer (2005). A Tn7-based broad-range bacterial cloning and expression system. Nature Methods, vol.2 NO.6, June 2005, 443.