Difference between revisions of "Part:BBa K592015"
Line 1: | Line 1: | ||
− | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K592015 short</partinfo> | <partinfo>BBa_K592015 short</partinfo> | ||
Line 5: | Line 4: | ||
This neatly designed color coding cassette is a prime candidate to replace BBa_J04450, the current RFP cassette in all of the BioBrick backbones. Bacteria colonies exhibit strong blue color after 18 hours. Due to the much darker blue/purple color tone, even partially blue colonies are readily recognized. | This neatly designed color coding cassette is a prime candidate to replace BBa_J04450, the current RFP cassette in all of the BioBrick backbones. Bacteria colonies exhibit strong blue color after 18 hours. Due to the much darker blue/purple color tone, even partially blue colonies are readily recognized. | ||
− | + | The promoter is constitutive, the RBS is strong, the double terminator is not B0015 but B0014. | |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 14:57, 18 September 2011
amilCP Blue Coding Device
This neatly designed color coding cassette is a prime candidate to replace BBa_J04450, the current RFP cassette in all of the BioBrick backbones. Bacteria colonies exhibit strong blue color after 18 hours. Due to the much darker blue/purple color tone, even partially blue colonies are readily recognized.
The promoter is constitutive, the RBS is strong, the double terminator is not B0015 but B0014.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]