Difference between revisions of "Part:BBa K553005:Design"

 
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<partinfo>BBa_K553005 short</partinfo>
 
<partinfo>BBa_K553005 short</partinfo>
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===Design Notes===
 
===Design Notes===
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ytft
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Assembling of a new biobrick BBa_K553002 (obtained by pcr from A.Tumefaciens gDNA), the RBS-glucosidase complex (BBa_J15001 + BBa_K392008 a gift from Dr. C. French, Edinbourg Univ.) and the double terminator BBa_B0015.
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===Source===
 
===Source===

Revision as of 14:29, 18 September 2011

C. firmi beta-glucosidase PTraI regulated


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 105
    Illegal XhoI site found at 1251
    Illegal XhoI site found at 1484
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 740
    Illegal AgeI site found at 183
    Illegal AgeI site found at 242
    Illegal AgeI site found at 636
    Illegal AgeI site found at 831
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 613


Design Notes

Assembling of a new biobrick BBa_K553002 (obtained by pcr from A.Tumefaciens gDNA), the RBS-glucosidase complex (BBa_J15001 + BBa_K392008 a gift from Dr. C. French, Edinbourg Univ.) and the double terminator BBa_B0015.

Source

uhuip

References