Difference between revisions of "Part:BBa K527001"

 
 
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<partinfo>BBa_K527001 short</partinfo>
 
<partinfo>BBa_K527001 short</partinfo>
  
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This BioBrick BBa_K527001 encode the plant enzyme Cytochrome P450 CYP79A2. Cytochromes p450 are defined as heme-thiolate proteins featuring a particular spectral signature at 450 nm, thus the name. Despite the label as cytochromes, these proteins are not involved in electron transfers, but act as monooxygenases in a wide range of reactions such as epoxidation, N-dealkylation, O-dealkylation, S-oxidation and hydroxylation.  
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This BioBrick BBa_K527001 encode the plant enzyme Cytochrome P450 CYP79A2. Cytochromes P450 are defined as heme-thiolate proteins featuring a particular spectral signature at 450 nm, thus the name. Despite the label as cytochromes, these proteins are not involved in electron transfers, but act as monooxygenases in a wide range of reactions such as epoxidation, N-dealkylation, O-dealkylation, S-oxidation and hydroxylation.  
  
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CYP79A2 is an N-hydroxylase converting L-phenylalanine into phenylacetaldoxime, the precursor of the cyanogenic glycoside benzylglucosinolate. Oximes as the phenylacetaldoxime is generally toxic to fungi and is frequently used as chemical fungicides. They function by inhibiting mitochondrial oxidases and thereby promote lipid peroxidation and the production of toxic reactive oxygen species.  
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CYP79A2 is an N-hydroxylase converting L-phenylalanine into phenylacetaldoxime, the precursor of a cyanogenic glycoside. Oximes as the phenylacetaldoxime is generally toxic to fungi and is frequently used as a chemical fungicide. They function by inhibiting mitochondrial oxidases and thereby promote lipid peroxidation and the production of toxic reactive oxygen species.  
  
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BBa_K527001 codes for the same enzyme as BBa_K527000, but is submitted in an other plasmid due to the different assembly method (Plug 'n' Play) hence the missing prefix and suffix.  
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BBa_K527001 codes for the same enzyme as BBa_K527000, but is submitted in an other plasmid (BBa_K678023) due to the different assembly method (Plug 'n' Play) hence the missing prefix and suffix.  
  
  
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<partinfo>BBa_K527001 parameters</partinfo>
 
<partinfo>BBa_K527001 parameters</partinfo>
 
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===References===
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* Wittstock U and Halkier B A (2000). ''Cytochrome P450 CYP79A2 from Arabidopsis thaliana L. Catalyzes the Conversion of L-Phenylalanine to Phenylacetaldoxime in the Biosynthesis of Benzylglucosinolate''. The Journal of Biological Chemistry Vol. 275, No. 19, Issue of May 12, pp. 14659–14666.
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* Møller B L (2010). ''Dynamic Metabolons.'' Science Vol. 330, 1328-1329.

Latest revision as of 14:18, 18 September 2011

Biosynthesis/Oximes/CYP79A2/Plug 'n' Play

This BioBrick BBa_K527001 encode the plant enzyme Cytochrome P450 CYP79A2. Cytochromes P450 are defined as heme-thiolate proteins featuring a particular spectral signature at 450 nm, thus the name. Despite the label as cytochromes, these proteins are not involved in electron transfers, but act as monooxygenases in a wide range of reactions such as epoxidation, N-dealkylation, O-dealkylation, S-oxidation and hydroxylation.

CYP79A2 is an N-hydroxylase converting L-phenylalanine into phenylacetaldoxime, the precursor of a cyanogenic glycoside. Oximes as the phenylacetaldoxime is generally toxic to fungi and is frequently used as a chemical fungicide. They function by inhibiting mitochondrial oxidases and thereby promote lipid peroxidation and the production of toxic reactive oxygen species.

BBa_K527001 codes for the same enzyme as BBa_K527000, but is submitted in an other plasmid (BBa_K678023) due to the different assembly method (Plug 'n' Play) hence the missing prefix and suffix.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 165
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 432


References

  • Wittstock U and Halkier B A (2000). Cytochrome P450 CYP79A2 from Arabidopsis thaliana L. Catalyzes the Conversion of L-Phenylalanine to Phenylacetaldoxime in the Biosynthesis of Benzylglucosinolate. The Journal of Biological Chemistry Vol. 275, No. 19, Issue of May 12, pp. 14659–14666.
  • Møller B L (2010). Dynamic Metabolons. Science Vol. 330, 1328-1329.