Difference between revisions of "Part:BBa K527002"
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<partinfo>BBa_K527002 short</partinfo> | <partinfo>BBa_K527002 short</partinfo> | ||
| − | This BioBrick BBa_K527002 encode the plant enzyme Cytochrome P450 CYP79B1. Cytochromes | + | This BioBrick BBa_K527002 encode the plant enzyme Cytochrome P450 CYP79B1. Cytochromes P450 are defined as heme-thiolate proteins featuring a particular spectral signature at 450 nm, thus the name. Despite the label as cytochromes, these proteins are not involved in electron transfers, but act as monooxygenases in a wide range of reactions such as epoxidation, N-dealkylation, O-dealkylation, S-oxidation and hydroxylation. |
CYP79B1 is an N-hydroxylase converting L-Tryptophan into indole 3-acetaldoxime, the precursor of a cyanogenic glycoside . Oximes as the indole 3-acetaldoxime is generally toxic to fungi and is frequently used as a chemical fungicide. They function by inhibiting mitochondrial oxidases and thereby promote lipid peroxidation and the production of toxic reactive oxygen species. | CYP79B1 is an N-hydroxylase converting L-Tryptophan into indole 3-acetaldoxime, the precursor of a cyanogenic glycoside . Oximes as the indole 3-acetaldoxime is generally toxic to fungi and is frequently used as a chemical fungicide. They function by inhibiting mitochondrial oxidases and thereby promote lipid peroxidation and the production of toxic reactive oxygen species. | ||
Revision as of 14:15, 18 September 2011
Biosynthesis/Oximes/CYP79B1/Plug 'n' Play
This BioBrick BBa_K527002 encode the plant enzyme Cytochrome P450 CYP79B1. Cytochromes P450 are defined as heme-thiolate proteins featuring a particular spectral signature at 450 nm, thus the name. Despite the label as cytochromes, these proteins are not involved in electron transfers, but act as monooxygenases in a wide range of reactions such as epoxidation, N-dealkylation, O-dealkylation, S-oxidation and hydroxylation.
CYP79B1 is an N-hydroxylase converting L-Tryptophan into indole 3-acetaldoxime, the precursor of a cyanogenic glycoside . Oximes as the indole 3-acetaldoxime is generally toxic to fungi and is frequently used as a chemical fungicide. They function by inhibiting mitochondrial oxidases and thereby promote lipid peroxidation and the production of toxic reactive oxygen species.
BBa_K527002 is submitted in the plasmid BBa_K678023 due to the different assembly method (Plug 'n' Play) hence the missing prefix and suffix.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]