Difference between revisions of "Part:BBa K527001:Design"

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===Design Notes===
 
===Design Notes===
−
Mutated at a Pst1 restiction site (nucleotide 342)
+
Mutated at a Pst1 restiction site (nucleotide 342).
 +
 
 
No prefix and suffix.
 
No prefix and suffix.
−
User assembly ends.
 
−
 
  
 +
User assembly ends (Plug 'n' Play).
  
 
===Source===
 
===Source===
  
 
Institute of Plant Biology and Biotechnology
 
Institute of Plant Biology and Biotechnology
 +
 
Faculty of LIFE Science
 
Faculty of LIFE Science
 +
 
University of Copenhagen
 
University of Copenhagen
 +
 
Denmark
 
Denmark
  
Line 22: Line 25:
  
 
===References===
 
===References===
−
* Wittstock U and Halkier B A (2000). ''Cytochrome P450 CYP79A2 from Arabidopsis thaliana L. Catalyzes
+
* Wittstock U and Halkier B A (2000). ''Cytochrome P450 CYP79A2 from Arabidopsis thaliana L. Catalyzes the Conversion of L-Phenylalanine to Phenylacetaldoxime in the Biosynthesis of Benzylglucosinolate''. The Journal of Biological Chemistry Vol. 275, No. 19, Issue of May 12, pp. 14659–14666.
−
the Conversion of L-Phenylalanine to Phenylacetaldoxime in the
+
−
Biosynthesis of Benzylglucosinolate''. The Journal of Biological Chemistry Vol. 275, No. 19, Issue of May 12, pp. 14659–14666.
+
  
 
* Møller B L (2010). ''Dynamic Metabolons.'' Science Vol. 330, 1328-1329.
 
* Møller B L (2010). ''Dynamic Metabolons.'' Science Vol. 330, 1328-1329.

Latest revision as of 13:51, 18 September 2011

Biosynthesis/Oximes/CYP79A2/Plug 'n' Play


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 165
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 432


Design Notes

Mutated at a Pst1 restiction site (nucleotide 342).

No prefix and suffix.

User assembly ends (Plug 'n' Play).

Source

Institute of Plant Biology and Biotechnology

Faculty of LIFE Science

University of Copenhagen

Denmark

http://2011.igem.org/Team:DTU-Denmark-2

References

  • Wittstock U and Halkier B A (2000). Cytochrome P450 CYP79A2 from Arabidopsis thaliana L. Catalyzes the Conversion of L-Phenylalanine to Phenylacetaldoxime in the Biosynthesis of Benzylglucosinolate. The Journal of Biological Chemistry Vol. 275, No. 19, Issue of May 12, pp. 14659–14666.
  • Møller B L (2010). Dynamic Metabolons. Science Vol. 330, 1328-1329.