Difference between revisions of "Part:BBa K539119"

Revision as of 08:19, 18 September 2011

crtY (lycopene cyclase)

This is the coding sequence of crtY from Pantoea ananatis (formerly Erwinia uredovora) (Accession number D90087). It encodes lycopene B-cyclase, part of the carotenoid biosynthesis pathway, which converts lycopene to B-carotene (Misawa, N., Nakagawa, N., Kobayashi, K., Yamano, S., Nakamura, K., and Harashima, K. 1990. Elucidation of the Erwinia uredovora carotenoid biosynthetic pathway by functional analysis of gene products expressed in Escherichia coli. Journal of Bacteriology 172, 6704-612).

We found a point mutation of BBa_K118008 (iGEM08_Edinburgh) at XbaI site and we corrected it successfully.(ACCTAGT->ACTAGT)

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 This is the coding sequence of crtY from Pantoea ananatis (formerly Erwinia uredovora) (Accession number D90087). It encodes lycopene B-cyclase, part of the carotenoid biosynthesis pathway, which converts lycopene to B-carotene (Misawa, N., Nakagawa, N., Kobayashi, K., Yamano, S., Nakamura, K., and Harashima, K. 1990. Elucidation of the Erwinia uredovora carotenoid biosynthetic pathway by functional analysis of gene products expressed in Escherichia coli. Journal of Bacteriology 172, 6704-612).
-We found a point mutation of BBa_K118008(iGEM08_Edinburgh) at XbalI site and we corrected it successfully.(ACCTAGT->ACTAGT)
+We found a point mutation of BBa_K118008 (iGEM08_Edinburgh) at XbaI site and we corrected it successfully.(ACCTAGT->ACTAGT)
 <!-- Add more about the biology of this part here