Difference between revisions of "Part:BBa K590023"
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<partinfo>BBa_K590023 short</partinfo> | <partinfo>BBa_K590023 short</partinfo> | ||
− | A mutated Kumamolisin-As enzyme aimed to combat | + | A mutated Kumamolisin-As enzyme aimed to combat gluten intolerance by increased activity with the PQLP peptide, an antigenic epitope in gliadin. |
===Usage and Biology=== | ===Usage and Biology=== |
Revision as of 20:09, 16 September 2011
Kumamolisin-As_N291D
A mutated Kumamolisin-As enzyme aimed to combat gluten intolerance by increased activity with the PQLP peptide, an antigenic epitope in gliadin.
Usage and Biology
To test BBa _K590023, it was inserted into a pET29b+ vector using [http://2011.igem.org/Team:Washington/Protocols/Kunkel Kunkel Mutagenesis]. Kumamolisin-As_N291D was then produced and purified as described in the [http://2011.igem.org/Team:Washington/Protocols/50mL_UW 2011 iGEM Team's Small Scale Protein Expression and Purification Protocol]. The purified protein was then tested for activity. For a detailed description of the assay, please see the [http://2011.igem.org/Team:Washington/Protocols/Purified_Enzyme_Assay 2011 UW iGEM Purified Enzyme Assay Protocol]. The resulting data is shown below.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1696
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 447
Illegal NgoMIV site found at 720
Illegal NgoMIV site found at 1248
Illegal NgoMIV site found at 1494
Illegal AgeI site found at 772
Illegal AgeI site found at 1348
Illegal AgeI site found at 1588 - 1000COMPATIBLE WITH RFC[1000]