Difference between revisions of "Part:BBa K590054"
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===Usage and Biology===
 
===Usage and Biology===
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[[Image:Washington_Redesign_Data2.png]]
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When AAR and ADC are co-expressed, they produce odd chain length alkanes from the cell's fatty acid biosynthetic pathway. Alkane production is enhanced when growing expression strains using the growth conditions developed by the [http://2011.igem.org/Team:Washington 2011 University of Washington iGEM Team]. 
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[[Image:Washinton_AARADCGC-MS.png|left|400px|thumb|GC plot showing that alkanes are only produced when both ADC and AAR are expressed.]]
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[[Image:Washinton_2011_Optimization_Quant.png|right|450px|thumb|GC-MS quantification shows greater alkane production under UW iGEM 2011's "optimized conditions" (see wiki for details)]]
  
  

Revision as of 19:37, 16 September 2011

RrD_N25M-PSB1C3-High constitutive

This part consists of Acyl-ACP Reductase, ribosomal binding site, mutant Aldehyde Decarbonylase under the control of high consititutive promoter for alkane production.

Usage and Biology

Washington Redesign Data2.png

When AAR and ADC are co-expressed, they produce odd chain length alkanes from the cell's fatty acid biosynthetic pathway. Alkane production is enhanced when growing expression strains using the growth conditions developed by the [http://2011.igem.org/Team:Washington 2011 University of Washington iGEM Team].

GC plot showing that alkanes are only produced when both ADC and AAR are expressed.
GC-MS quantification shows greater alkane production under UW iGEM 2011's "optimized conditions" (see wiki for details)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Unknown
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1584
    Illegal XhoI site found at 2366
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 126
  • 1000
    COMPATIBLE WITH RFC[1000]