Difference between revisions of "Part:BBa K546001:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | The original R0063 BioBrick part (promoter) of the F2621 BioBrick device turned out to be absent in our construct, as was shown by a sequence analysis. To increase the transcription activation | + | The original R0063 BioBrick part (promoter) of the F2621 BioBrick device turned out to be absent in our construct, as was shown by a sequence analysis. To increase the transcription activation, an (extra) R0063 BioBrick part promoter was placed in front of the F2621 BioBrick device. |
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===Source=== | ===Source=== |
Revision as of 18:20, 16 September 2011
Lux pL controlled luxR with lux pR controlled AHL degrading enzyme AiiA(lva tag).
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1283
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1101
Design Notes
The original R0063 BioBrick part (promoter) of the F2621 BioBrick device turned out to be absent in our construct, as was shown by a sequence analysis. To increase the transcription activation, an (extra) R0063 BioBrick part promoter was placed in front of the F2621 BioBrick device.
Source
The lux p promoters originate from the quorum sensing ability of Vibrio fischeri.