Difference between revisions of "Part:BBa K538003"

 
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<partinfo>BBa_K538003 short</partinfo>
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===CspA (''Polaribacter irgensii'')===
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[[Image:Csp_graph.jpg||right|frame|Figure 1: Freeze/thaw cycle survival rate of ''E. coli'' containing an empty vector (black circles), a vector carrying ''P. irgensii'' 's ''CspA'' gene (white circles), and a vector carrying ''P. irgensii'' 's ''CspC'' gene (white triangles). '''Adapted from Uh ''et al.'' (2010)''']]
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This BioBrick encodes the ''CspA'' protein of the Arctic cryophile ''Polaribacter irgensii''. It's homologous to ''E. coli'' 's cold-induced RNA chaperones ''CspA'' and ''CspD'', and highly similar to ''P. irgensii'''s ''CspC'' ([[Part:BBa_K538004|K538004]]).  Uh ''et al.'' managed to increase the freeze/thaw cycle survival rate of ''E. coli'' more than five-fold by overexpressing this protein. (Figure 1)
  
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===Usage===
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
 
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<partinfo>BBa_K538003 SequenceAndFeatures</partinfo>
 
  
  
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===Biology===
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===Functional Parameters===
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<partinfo>BBa_K538003 parameters</partinfo>
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===Characterisation===
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'''[Update pending]'''
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===Safety===
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There are risks involved with enhancing bacterial cold resistance and facilitating their growth at low temperatures. Please refer to the [http://2011.igem.org/Team:Amsterdam/Project/Safety safety page] of this brick's developers.
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===References===
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===Sequence and Features===
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<partinfo>BBa_K538003 SequenceAndFeatures</partinfo>

Revision as of 11:03, 16 September 2011

CspA (Polaribacter irgensii)

Figure 1: Freeze/thaw cycle survival rate of E. coli containing an empty vector (black circles), a vector carrying P. irgensii 's CspA gene (white circles), and a vector carrying P. irgensii 's CspC gene (white triangles). Adapted from Uh et al. (2010)

This BioBrick encodes the CspA protein of the Arctic cryophile Polaribacter irgensii. It's homologous to E. coli 's cold-induced RNA chaperones CspA and CspD, and highly similar to P. irgensii's CspC (K538004). Uh et al. managed to increase the freeze/thaw cycle survival rate of E. coli more than five-fold by overexpressing this protein. (Figure 1)


Usage

Biology

Characterisation

[Update pending]


Safety

There are risks involved with enhancing bacterial cold resistance and facilitating their growth at low temperatures. Please refer to the [http://2011.igem.org/Team:Amsterdam/Project/Safety safety page] of this brick's developers.


References

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]