Difference between revisions of "Part:BBa K523022"
Line 3: | Line 3: | ||
Lac promoter driving expression of crtE, crtI, and crtB (carotenoid synthesis pathway). | Lac promoter driving expression of crtE, crtI, and crtB (carotenoid synthesis pathway). | ||
+ | |||
+ | Part was assembled as a demonstration of Edinburgh's 2011 BioSandwich protocol. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 20:45, 15 September 2011
PlacLacZ + crtEIB
Lac promoter driving expression of crtE, crtI, and crtB (carotenoid synthesis pathway).
Part was assembled as a demonstration of Edinburgh's 2011 BioSandwich protocol.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1651
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 3665
Illegal NgoMIV site found at 3795
Illegal AgeI site found at 1346 - 1000COMPATIBLE WITH RFC[1000]