Difference between revisions of "Part:BBa K639003"
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<partinfo>BBa_K639003 short</partinfo> | <partinfo>BBa_K639003 short</partinfo> | ||
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The stringent response in bacteria is caused by amino-acid starvation, fatty acid limitation, iron limitation, heat shock and other stress conditions. As a response under these conditions, ''in vitro'' studies have suggested that the alarmone guanosine tetraphosphate (ppGpp) increase to modulate transcription to promote survival. The increase in ppGpp levels causes a redirection of transcription so that genes important for survival are favoured at the expense of those required for growth and proliferation [1]. | The stringent response in bacteria is caused by amino-acid starvation, fatty acid limitation, iron limitation, heat shock and other stress conditions. As a response under these conditions, ''in vitro'' studies have suggested that the alarmone guanosine tetraphosphate (ppGpp) increase to modulate transcription to promote survival. The increase in ppGpp levels causes a redirection of transcription so that genes important for survival are favoured at the expense of those required for growth and proliferation [1]. | ||
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[[http://www.sciencedirect.com/science/article/pii/S0966842X05000788 1]] Magnusson, L. U., A. Farewell, et al. (2005). "ppGpp: a global regulator in Escherichia coli." Trends Microbiol 13(5): 236-242 | [[http://www.sciencedirect.com/science/article/pii/S0966842X05000788 1]] Magnusson, L. U., A. Farewell, et al. (2005). "ppGpp: a global regulator in Escherichia coli." Trends Microbiol 13(5): 236-242 | ||
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[[File:rrnB+LacI+pLac+mCherry_rosa.png|780px]] | [[File:rrnB+LacI+pLac+mCherry_rosa.png|780px]] |
Revision as of 15:33, 15 September 2011
rrnB P1-LacI-pLac-mCherry plausible stress sensor
File:Construct map 1807.jpg
Plasmid map of the complete construct. Promoters are shown in blue, and genes as inside arrows. Only single restriction sites are shown.
Biobrick | Part number |
---|---|
LacI with RBS | BBa_J24679 |
Terminator | BBa_B0015 |
pLac | BBa_R0011 |
mCherry with RBS and terminator | BBa_J06702 |
The promotor is a variant of BBa_K112118, that we have made ourselves using PCR, and the PCR product was directly used as insert and connected with the rest of the construct. The final construct was cut with BstBI to verify that the rrnB P1 had been insertet. The stress sensor has a total length of 2653 bp, and is located inside the plasmid pSB1A2, carrying ampicillin resistance. We have put the stress sensor through several tests (see [http://2011.igem.org/Team:NTNU_Trondheim/stress-sensor characterization])
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1666
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]