Difference between revisions of "Part:BBa K525405:Design"
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** lac-operator from ''E. coli'' | ** lac-operator from ''E. coli'' | ||
| − | * <partinfo>K525401</partinfo> S-layer gene ''sbpA'' synthesized, originated in ''Lysinbacillus sphaericus'' | + | * <partinfo>K525401</partinfo> S-layer gene ''sbpA'' synthesized, originated in ''Lysinbacillus sphaericus'' CCM 2177 |
* <partinfo>J18931</partinfo> yellow fluorescent protein (YFP) mCitrine | * <partinfo>J18931</partinfo> yellow fluorescent protein (YFP) mCitrine | ||
** from parts.igem | ** from parts.igem | ||
** was synthesized before it was sent in | ** was synthesized before it was sent in | ||
| − | |||
===References=== | ===References=== | ||
Revision as of 11:28, 15 September 2011
Fusion Protein of S-Layer SbpA and mCitrine
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 104
Illegal BglII site found at 221
Illegal XhoI site found at 1996 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 76
Illegal AgeI site found at 3913 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 493
Illegal BsaI.rc site found at 622
Design Notes
- BBa_K525403 fused to BBa_J18931
- BBa_K525403 synthesized, codon optimized and in Freiburg assembly standard (RFC 25) to easily create fusion proteins
- NgoMIV restriction site downstream the start codon to easily fuse an N-terminal domain to the coding sequence
- AgeI restriction site upstream of stop codon to easily fuse a C-terminal domain to the coding sequence
Source
- BBa_K525403 fused to BBa_J18931
- BBa_K525403 synthesized, codon optimized and in Freiburg assembly standard (RFC 25) to easily create fusion proteins
- Promoter: fusion promoter between T7 promoter and lac-operator
- T7 promoter from T7 phage
- lac-operator from E. coli
- BBa_K525401 S-layer gene sbpA synthesized, originated in Lysinbacillus sphaericus CCM 2177
- BBa_J18931 yellow fluorescent protein (YFP) mCitrine
- from parts.igem
- was synthesized before it was sent in
References
Kainz B, Steiner K, Möller M, Pum D, Schäffer C, Sleytr UB, Toca-Herrera JL (2010) Absorption, Steady-State Fluorescence, Fluorescence Lifetime, and 2D Self-Assembly Properties of Engineered Fluorescent S-Layer Fusion Proteins of Geobacillus stearothermophilus NRS 2004/3a, [http://pubs.acs.org/doi/abs/10.1021/bm901071b Biomacromolecules 11(1):207-214].
Sleytr UB, Huber C, Ilk N, Pum D, Schuster B, Egelseer EM (2007) S-layers as a tool kit for nanobiotechnological applications, [http://onlinelibrary.wiley.com/doi/10.1111/j.1574-6968.2006.00573.x/full FEMS Microbiol Lett 267(2):131-144].