Difference between revisions of "Part:BBa K568001:Design"
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Designed from existing Biobricks. | Designed from existing Biobricks. | ||
| − | High Fidelity PCR was performed on K322127 (Red Light Sensor Part with lacZ promoter) to amplify the region withouth RBS and LacZ. The PCR product contains pcyA, heme oxygenase, as well as CPH8 and ompC promoter (correspond to parts K322123, K322124 and R0082, which form BBa_K568000). This PCR product was assembled with a synthesized construct containing the small parts of the AND- | + | High Fidelity PCR was performed on K322127 (Red Light Sensor Part with lacZ promoter) to amplify the region withouth RBS and LacZ. The PCR product contains pcyA, heme oxygenase, as well as CPH8 and ompC promoter (correspond to parts K322123, K322124 and R0082, which form BBa_K568000). This PCR product was assembled with a synthesized construct containing the small parts of the AND-Gate (BBa_K568006). The final step was ligating this intermediate part BBa_K568005 with T7ptag (BBa_K228000). |
===References=== | ===References=== | ||
Revision as of 21:11, 14 September 2011
Optogenetical AND-Gate red/blue light
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 644
Illegal XhoI site found at 1786 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 971
Illegal AgeI site found at 3817 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 3820
Design Notes
We used YcgF/E as blue light sensor and Cph8 as red light sensor. We expect that this assembly will be the one with the lowest amount of unspecific gene expression which could occur due to overlapping of the absorption spectrums of the sensory domains.
Source
Designed from existing Biobricks.
High Fidelity PCR was performed on K322127 (Red Light Sensor Part with lacZ promoter) to amplify the region withouth RBS and LacZ. The PCR product contains pcyA, heme oxygenase, as well as CPH8 and ompC promoter (correspond to parts K322123, K322124 and R0082, which form BBa_K568000). This PCR product was assembled with a synthesized construct containing the small parts of the AND-Gate (BBa_K568006). The final step was ligating this intermediate part BBa_K568005 with T7ptag (BBa_K228000).