Difference between revisions of "Part:BBa K568001"

Revision as of 21:07, 14 September 2011

Optogenetical AND-Gate red/blue light

This logical gate is based on amber stop-codon suppression via the non-canonical tRNA supD(Ba_K228001). The blue light sensor(BBa_K238013) induces the expression of a mRNA coding for a T7-polymerase with an amber mutation(BBa_K228000), that can only be translated by ribosomes if the correct supD tRNA is present. The supD tRNA is expressed when the red light sensor(BBa_K568000) is induced. Thus, only cells which receive both signals produce the desired substances.

Usage and Biology

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 644
Illegal XhoI site found at 1786
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 971
Illegal AgeI site found at 3817
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 3820

Functional Parameters

n/a	Optogenetical AND-Gate red/blue light

Revision as of 21:06, 14 September 2011 (view source) Thorsten mueller (Talk \| contribs) ← Older edit		Revision as of 21:07, 14 September 2011 (view source) Thorsten mueller (Talk \| contribs) Newer edit →
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	<partinfo>BBa_K568001 short</partinfo>		<partinfo>BBa_K568001 short</partinfo>

−	This logical gate is based on amber stop-codon suppression via the non-canonical tRNA supD(Ba_K228001). The blue light sensor(BBa_K238013) induces the expression of a mRNA coding for a T7-polymerase with an amber mutation(BBa_K228000), that can only be translated by ribosomes if the correct supD tRNA is present. The supD tRNA is expressed when the red light sensor(<html><a href="https://parts.igem.org/Part:BBa_K568000">~~aaaaaa~~</a></html>) is induced. Thus, only cells which receive both signals produce the desired substances.	+	This logical gate is based on amber stop-codon suppression via the non-canonical tRNA supD(<html><a href="https://parts.igem.org/Part:Ba_K228001">Ba_K228001</a></html>). The blue light sensor(BBa_K238013) induces the expression of a mRNA coding for a T7-polymerase with an amber mutation(<html><a href="https://parts.igem.org/Part:BBa_K228000">BBa_K228000</a></html>), that can only be translated by ribosomes if the correct supD tRNA is present. The supD tRNA is expressed when the red light sensor(<html><a href="https://parts.igem.org/Part:BBa_K568000">BBa_K568000</a></html>) is induced. Thus, only cells which receive both signals produce the desired substances.