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==Batch Notes==
 
==Batch Notes==

Revision as of 15:02, 14 September 2011

Batch Summary

Use this page to keep track of competent cell production. See the competent cell protocol for more information.

Batch Strain Maker Date Started Temp, RPM Incubation (hr) Culture Volume Culture OD (600nm) Competent OD (600nm) Cell Count (AVG) Efficiency 250ul Aliquots 500ul Aliquots 1ml Aliquots 96w Plate
Expected Values20C160.3 or lower 1 - 1.5100 - 4005*10^8 - 5*10^9
001 NEB 10b VS+KN 8/17/11 21C, 250rpm 16 500ml 0.4 1.35 67,88,112 (89) 1.335*10^8 20 20 10 0
002 NEB 10b VS+KN 9/12/11 22C, 250rpm 16 500ml 0.40 ?? ??, ??, ?? (??) ?? ?? 0 4
003 NEB 10b VS+KN 9/12/11 22C, 250rpm 16 500ml 0.23 ?? ??, ??, ?? (??) ?? ?? 0 4

Batch Notes

001

This is the first batch attempting to fully standardize competent cell production. Also Kitwa's first batch! And a resounding success!

The efficiency test/test transformation used a single 250ul aliquot (randomly chosen). Three transformations were done using our standard template and protocol. Important: In following productions we will test the first and last of the aliquots.