Difference between revisions of "Part:BBa K608404:Design"

 
(→‎References)
 
(One intermediate revision by the same user not shown)
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K608404 short</partinfo>
 
<partinfo>BBa_K608404 short</partinfo>
Line 13: Line 12:
 
===Source===
 
===Source===
  
−
reference: "The nature of target-unrelated peptides recovered in the screening
+
===References===
 +
"The nature of target-unrelated peptides recovered in the screening
 
of phage-displayed random peptide libraries with antibodies"
 
of phage-displayed random peptide libraries with antibodies"
−
 
−
===References===
 

Latest revision as of 12:23, 14 September 2011

IPTG-inducible Promoter with plastic binding domain-tagged GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Unknown
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 870


Design Notes

To make the plastic binding domain observable we coupled it to GFP as a reporter. Due to the high hydropathy of the plastic binding sequences this protein seems to be toxic to the bacteria. For this reason we used an inducible promoter for the expression of this part. The cells can be cultivated until an optical density of 60-80 followed by an addition of IPTG to induce the expression of the protein.


Source

References

"The nature of target-unrelated peptides recovered in the screening of phage-displayed random peptide libraries with antibodies"