Difference between revisions of "Part:BBa K608404:Design"

 
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===Source===
 
===Source===
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reference: "The nature of target-unrelated peptides recovered in the screening
 
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of phage-displayed random peptide libraries with antibodies"
 
  
 
===References===
 
===References===

Revision as of 12:23, 14 September 2011

IPTG-inducible Promoter with plastic binding domain-tagged GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Unknown
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 870


Design Notes

To make the plastic binding domain observable we coupled it to GFP as a reporter. Due to the high hydropathy of the plastic binding sequences this protein seems to be toxic to the bacteria. For this reason we used an inducible promoter for the expression of this part. The cells can be cultivated until an optical density of 60-80 followed by an addition of IPTG to induce the expression of the protein.


Source

References